Immunomodulatory and hematoprotective potential of withania somnifera aqueous root extract (wre) against subacute toxicity of monocrotophos in commercial broilers Gupta Shefali1,*, singh S.P.2, saheb M.2, Ahmad A.H.2 1Veterinary Officer, Govt Vety Hopital Diagnosis LaboratoryBabugarh, Hapur, U.P. 2Departmant of Pharmacology & Toxicology, College of Veterinary and Animal Sciences, G. B. Pant Univ of Ag & Technology, Pantnagar-263145 *Correspding author email: gshefu@gmail.com
Online published on 8 April, 2021. Abstract The objective of the present investigation was to access immuno protective and hematoprotectiveeffect of Withania somnifera against subacute toxicity of monocrotophos (MCP) in chickens. The study consisted of 5 groups having 12 birds in each. Monocrotophos (2 ppm in feed daily) was administered to birds of group II to V and Withania somnifera aqueous root extract (100, 300 and 500 mg/kg b. wt /day) was given in drinking water to birds of group III, IV and V birds respectively for 6 weeks. Group I served as control.MCP decreased the levels of Hb, TEC, TLC, total protein, globulin, lymphocytes and AChE activity while increased heterophil, creatinine, cholesterol, AST, ALT, ALP levels in exposed birds. These hemato-biochemical changes were ameliorated by Withania somniferain WRE+MCP-treated birds after 4 and 6 weeks. Activity of AChE (in brain and RBC) became almost normal in WRE-treated birds. ZnSO4 turbidity test, ELISA and LST showed decrease in total Immunoglobulin (Ig) level, antibody titer and “OD of B and T cells in MCP-exposed group II birds, respectively. Increase in total Ig levels were recorded in extract-treated chickens of group IV and V while antibody titre increased in dose dependent manner in WRE-treated group III – V. WRE @ 500mg/kg significantly increased LPS stimulated cultures of B lymphocytes and @ 300 and 500 mg/kg increased PHA-stimulated cultures of T lymphocytes, respectively.MFT and DTH revealed decrease in % NBT positive macrophages and skin thickness, respectively in MCP-exposed group II birds. WRE significantly improved % NBT positive macrophages @ 300 and 500 mg/kg b. wt. Also, significant increase in skin thickness was observed following second exposure of dinitrofluorobenzene in chickens of extract treated group V at 6, 12, 24 and 72 h intervals and in group IV at 24 h interval. These observations suggested protective role of Withania somnifera aqueous root extract against MCP-induced immunosuppression and hematobiochemical alterations. Top Keywords Monocrotophos, Immunosuppression, Immunomodulation, Withania somnifera, Chickens. Top |