Growth rate and antibiotic sensitivity effect of some natural and petroleum based materials on Staphylococcus aureus Yahya Esam Bashir1*, Alfallous Khalifa A.2, Wali Asma3, Hameid Sohair4, Zwaid Hanah5 1Senior Lecturer, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya 2Associated Professor, Department of Chemistry, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya 3Student, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya 4Student, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya 5Student, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya * Corresponding Author: essam912013@gmail.com
Online published on 4 January, 2021. Abstract Numerous bacteria expose to different materials every day. Bacterial genome mainly composed of a single double-stranded circular DNA molecule, which can easily undergo changes or mutations upon the exposure to many substances. The aim of this study was to evaluate the mutagenic effect in term of growth rate and antibiotic sensitivity of some natural and petroleum based materials on Staphylococcus aureus. Exposure to each of Bunsen and acetone lower the growth rate of bacterial cells compared to diesel and engine oil that dramatically stimulate their growth. Tobacco based products and the low concentrations of tea and coffee accelerate the growth. The high concentrations of caffeine inhibit the bacterial growth. Wild type bacteria was sensitive to most of used antibiotic and gained resistance to many of them after the exposure to the petroleum products. Similarly, tobacco and tea, which accelerate the growth of cells, make them also completely resistant to the antibiotics that inhibit the synthesis of cell walls. Based on the obtained results, it can be concluded that even natural products can induce bacterial gene mutations such as antibiotic resistance. Top Keywords Mutagenic, Exposure, Bacteria, Antibiotic Resistance Growth Rate. Top |