Annotation of plant genome: A case study of Oryza sativa Kaur Harpreet1*, Keshri Rajan2, Mir Tanzeel Tufail3 1Research Scholar, Guru Arjan of Dev Institute Development Studies, 14-Preet Avenue, Majitha Road, PO Naushera, Amritsar-143008, India 2Research Intern, Guru Arjan of Dev Institute Development Studies, 14-Preet Avenue, Majitha Road, PO Naushera, Amritsar-143008, India 3Research Intern, Guru Arjan of Dev Institute Development Studies, 14-Preet Avenue, Majitha Road, PO Naushera, Amritsar-143008, India * Corresponding Author: harpreetkaur1182@yahoo.com
Online published on 4 January, 2021. Abstract Rice A perennial claim crop of the world. Besides satisfying the eagerness of energy rice, has also been known to support the world's trade economy. Hence, being a crop of such crucial importance its examinational study at the genome level will serve in multiplying its production and quality to irrigate the burning crave of humanity. Likewise, the senescence gene of rice is responsible for its age duration. Hence, understanding its property at 360° will help us to modify or to alter its function in a positive portion. Using Insilco analysis mode, the present study is an attempt to examine various characteristics conformation of senescence causing genes in rice. The two genes chosen were HCP and RR because the interaction between these two led to the onset of senescence in rice. Two genes that are HCP (Histidine-containing phosphotransfer protein 1) and RR (Two-component response regulator) are responsible for attaining the stage of senescence in rice. Understanding their molecular and structural property will be going to let us closer to perform successful adjustments. Moreover, their specific property is also responsible for their specific interaction which led to the generation of such signals that triggers senescence. Therefore, this analysis was aimed to understand the features of the two genes as well as their interaction by the means of computational technique. Understanding the features, function and flow of gene will lead us to stabilized effective measures in order to get a beneficiary outcome while going for alteration in its characters. As the pure data for the structural conformation of the selected genes are not available so, we have at first, searched the most similar homolog of the query sequence and the search was based on similar sequence homology on the platform of the local alignment tool. And the further analysis was carried out on the base conformation of the most relevant homologs (structure/sequence) found. We have analyzed the query gene sequence by various dry lab analysis tools to explore its structural and molecular features with the motive to contribute a little knowledge for the sake of further studies to delay senescence in rice plants in order to increase grain productivity. Top Keywords Senescence, Interaction, Signals, Plant Genome Annotation, Plant Ontologies, Plant Gene Family Data Bases, Genome Annotation Pipelines, Functional Annotation, Annotation Repetitive Sequence Functional Description. Top |