Skin sensitization assay of topical occluded heat patches by modified buehler method using Rabbits Salmam Muhi N. Dr.* Department of Toxicology, Baghdad College of Medical Sciences, Baghdad, Iraq * Corresponding Author: dr.damuk-sb@hotmail.com
Online published on 4 January, 2021. Abstract Background Many heat patches are known, based on a reaction system comprising iron, carbon, vermiculite, potassium chloride, and water. The heat release from the inorganic oxidation of iron is initiated by exposure to oxygen, such as when the patch is removed from the plastic pouch. Severe burns have occurred when these products are used in conjunction with a heat source such as an analgesic heating patches. Objectives Concepts in Cutaneous toxicity is to determine the potential of heat patches to promote skin sensitization reactions after repeated applications using the although modified Buehler test protocols were used. Buehler tests were conducted according to OECD guideline #406 using a multiple-dose design and test results were analyzed using a standard logistic dose-response model. Contact dermal sensitization is an immunological process where the host animal, through repeated skin exposure, acquires a specific allergic sensitivity to heat patches. In the modified Buehler model, using rabbit contact dermal sensitivity is manifested as increased erythema and /or oedema. Materials and Methods In the modified using rabbits Buehler protocol test, the induction group at 24 hours after challenge patch removal and again at 48 hours after patch removal with induction patches (1 patch per week). Two weeks after induction the animals are challenged by closed-patch tests to the rabbit's abdominal wall skin for 6h. All tests in the Buehler assay are performed on restrained animal we investigated the feasibility of using heat patches topically applied to abdominal wall skin (rabbits) clipped free of hair by experimental induction and challenge phases as an acceptable method to assess the potential of a occluded exposure to analgesic heat patches to promote skin sensitization reaction. Ressuts Depending on collected tables data of challenge versus rechallenge weeks (1,2,7) senisation reaction results, shown that the potential cutaneous toxicity of the test heat patches was non -irritating to week sensitizer category using rabbit's abdominal wall skintopical exposure in the test animals by the modified Buehler test. Conclusion The safety use of topical occluded heat patches as pain analgesic if the end-user follows the instruction of application properly. Top Keywords Dose response, Guinea pig, OECD guideline, Allergic contact dermatitis, Contact Rabbits abdominal wall. Top |