Immunotoxic effects of acetamiprid following subchronic exposure in swiss albino mice Preeti, Jain S.K.*, Lather Deepika1 Department of Veterinary Pharmacology and Toxicology 1Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana *Corresponding author e-mail: preetisuhag.vets@gmail.com
Online published on 8 April, 2021. Abstract Acetamiprid, a neonicotinoid insecticide has been in extensive use worldwide for several years in agriculture and veterinary medicine. The objective of the present study was to evaluate immunotoxic effects of acetamiprid following subchronic exposure in male Swiss albino mice. Acetamiprid was administered i.p. daily at 4.6 and 2.3 mg/kg/day along with 3% gum acacia as negative control for 60 and 90 days. Specific parameters of humoral and cellular immune response including hemagglutinating antibody (HA) titer to sheep red blood cells (SRBC; T-dependent antigen) and delayed type hypersensitivity (DTH) response to SRBC was evaluated. Histopathology of spleen and footpad was also done. The result showed that acetamiprid suppressed both humoral and cell mediated immune response as evident from decrease in HA titer and decrease in DTH response. In 60 days treatment groups, significant decrease in HA titer and DTH response was only observed at 4.6 mg/kg/day. In 90 days treatment groups, significant decrease in HA titer and DTH response was observed at 4.6 and 2.3 mg/kg/day from control group. At these dose levels, there were prominent histopathological alterations in spleen and footpad sections. The results indicated that acetamiprid has immunosuppressive effects at both dose levels which could potentially be attributed to direct cytotoxic effects of acetamiprid against T cells and that long term exposure could be detrimental to the immune system. Top Keywords Immunotoxicity, Acetamiprid, Swiss albino mice, Hemagglutinating antibody (HA) titer, Delayed type hypersensitivity (DTH) response. Top |