Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2003, Volume : 24, Issue : 2
First page : ( 153) Last page : ( 154)
Print ISSN : 0970-9320.

Diagnosis of Mycoplasma mycoides subsp. mycoides type lc infection by polymerase chain reaction

Kumar Manoj, M., Singh V.P.*, Srivastava N.C., Sharma Bhaskar

National Referral Laboratory on Mycoplasma, Division of Bacteriology & Mycology Indian Veterinary Research Institute, Izatnagar-243122 (U.P.)

*Corresponding authors

Received:  21  February,  2004.

Abstract

PCR assay was undertaken for rapid diagnosis of caprine pleuropneumonnia caused by Mycoplasma mycoides subsp . mycoides type LC directly from the experimental clinical materials without prior isolation of the organisms. Specific amplifications were observed with M. mycoides cluster specific, M. mycoides group specific and M. mycoides subsp . mycoides LC specific primers confirming the suitability of PCR for rapid diagnosis of mycoplasma infections by overcoming the problems associated with their isolation.

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