Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2003, Volume : 24, Issue : 2
First page : ( 149) Last page : ( 152)
Print ISSN : 0970-9320.

Characterisation of an indian isolate of turkey pox virus

Singh Alka, Dash B.B., Kataria J.M., Dandapat S., Dhama K.

Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar–243 122 (U.P.)

Received:  24  May,  2004.

Abstract

A turkey poxvirus isolated from a natural outbreak of pox in turkeys was characterised using different physico-chemical agents and transmission electron microscopy (TEM) technique. The complete inactivation of the virus was achieved at 60°C for 30 min and with the chemicals like trypsin (0.25%), phenol (0.5%) and chloroform (5%). The turkey pox virus isolate was found sensitive when exposed to the temperature of 50°C for 30 min and to the chemicals like diethyl ether (20%) and formalin (0.5%) as the infectivity of the virus was reduced up to a considerable extent after its exposure to these agents. The electron microscopic features of the turkey pox virus was studied by examining the CAM of infected chicken embryos as well as scabs collected from turkeys infected with turkey pox virus after negative staining and the virus showed typical pox virus morphology as having characteristic oval shaped structure with dumble shaped dense core, surrounded by a double layer membrane and was found free in cytoplasm of the cell.

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