Enzymatic studies with reference to antispermatogenic efect of 5thio-D- glucose on testicular metabolism of albino rat Changamma C.*, Lakshman J., Basha S. Hasim, Naik A Govardhan Department of Zoology, S V University P G Centre,KAVALI-524 201 (A.P.), India *Email: challa_changamma@yahoo.co.in
Online published on 22 February, 2013. Abstract 5-Thio-D-glucose is chemically analogous of biologically important D-glucose, competitively inhibits transport of D-glucose across cell membrane. The treatment produces germinal cell degeneration and complete inhibition of spermatogenesis in the seminiferous tubules. The antifertility action of 5-Thio-D-glucose on male is associated with a marked reduction in the ability of spermatozoa through oxidize glucose. In view of the facts that testis depends more on carbohydrate reserves for its energy and 5-Thio-D-glucose competitively inhibits the carbohydrate metabolism. Hence it will be interesting to study the same in testis during 5-Thio-D-glucose treatment. In the present study adult male Wistar strain albino rats were administered (sc) 5-Thio-D-glucose for a period of 7 days and the enzyme levels were analyzed in testes. The changes in gravimetric analysis indicate the alterations in testicular biochemical constituents. The study on tissue proximate analysis shows the impaired carbohydrate and lipid metabolism. The decreased carbohydrates suggest the orientation towards glycogenesis, glycolysis and increased gluconeogenesis utility resulting in decreased glycogen and glucose content. Since the germ cells depend more on carbohydrate reserves, the decreased carbohydrate reserves might be due to observed reduction of spermatids and spermatozoal population. The decreased levels of G-6-PDH suggest the reduced operation of HMP pathway leads to deranged lipogenesis and nucleic acid synthesis. Depleted levels of NAD+ -GDH suggest the decreased amino acid oxidation. The differential responses of SDH and MDH may resulting in the activation and inactivation of certain segments of citric acid cycle through different cofactors like FAD+ and NAD+. Thus 5-Thio-D-glucose induces the HMP pathway and certain enzymes of citric acid cycle which in turn shows the effect on oxidative metabolism leads to impaired steroidogenesis. Top Keywords antifertility, Glycogenesis, Gluconeogenesis, Lipogenesis, Steroidogenesis, HMP pathway. Top |