Study of gonado-somatic index and protein fractions by sds-page in ovaries of spodoptera exigu Tomar Deepti*, Bhide Mangla Department of Zoology, Dr. H. S. Gour University, Sagar – 470002, (M. P.) *Email: diptitomar24@gmail.com; tomardipti7@yahoo.com
Online published on 22 February, 2013. Abstract The parameter of GSI plays very significant role which evaluate the reproductive potency of the concerning animal. Flavonol and quercitrin glycoside showed adverse effect on the GSI (FFI) hence directly or indirectly affecting the fertility of Spodoptera exigua in the present investigation. Deviation from normal or control and increase in GSI indicating the oedematous condition or cancerous growth or hyperactivity of gonads while decrease in GSI indicating poor development, inactiveness of gonads and malformation of gametes. The depletion, destruction and degeneration of protein metabolites in the gonads of experimental groups of Spodoptera exigua, correlated with the depletion of –vely charged protein fractions were detected by SDS-PAGE. Ovarian samples of female Spodoptera exigua consists of variable protein fractions ranging from 50 kDa of high intensity in 2 day, 190 kDa of high intensity in 4 day, 115 kDa of weak intensity in 6 day, 190 kDa of high intensity in 8 day flavonol glycoside treated insects and 50–75 kDa of high intensity in 2 day, 50–190 kDa of weak intensity in 4 day, 195 kDa of high intensity in 6 day, 195 kDa of high intensity in 8 day quercitrin glycoside treated insects. A rapid cessation of oviposition was noted due to detrimental oocyte growth in 2nd -8th days flavonol glycoside treated adult female. No significant reduction in quercitrin glycoside was observed as compared to flavonol glycoside. Results indicate that had a strong toxicity on adult female of Spodoptera exigua than quercitrin glycoside treated adult female. Top Keywords GSI, FFI, Spodoptera exigua, Flavonol glycoside, Quercitrin glycoside. Top |