Year : 2009, Volume : 33, Issue : 2
First page : ( 143) Last page : ( 147)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2009 December 1.

Pathogenicity study of Indian isolates of infectious bronchitis virus in chickens

Bhuvaneswari T.¹, Singh S. D.^1,*

¹Avian Diseases Section, Division of Pathology Indian Veterinary Research Institute, Izatnagar-243122

*Corresponding author; e-mail: sdsingh2005@rediffmail.com

Abstract

In the present study four field isolates of Infectious bronchitis virus (IBV), India/627/AD/02, India/744/AD/04, India/764/AD/04 and India/16/V/AD/07, of different geographical origin were adapted in 9–11 day-old embryonated chicken eggs(ECE). The infected embryos appeared curled with deformed feet and compressed over the head. Urate deposition in eggs infected with isolates India/764/AD/04 and India/16/V/AD/07 was noticed. The EID₅₀ titre of four IBV isolates ranged from 10⁵³ to 10⁶⁴ EID₅₀. Experimental infection in chicks revealed that all four isolates induced clinical symptoms and post mortem lesions confined to respiratory epithelium. The isolates India/764/AD/04 and India/16/V/AD/07 induced nephritis and urate deposit suggesting the involvement of nephropathogenic strains of IBV. The isolate India/627/AD/02 was found highly pathogenic to respiratory epithelium followed by India/764/AD/04, India/744/AD/04 and India/16/V/AD/04.The isolate India/764/AD/04 was found highly nephropathogenic and also caused respiratory infections. IBV antigen was detected in different organs like trachea, lungs, kidneys and caecal tonsils by indirect FAT.

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Keywords

Chicken, Infectious bronchitis, Nephropathogenic, Pathogenicity.

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