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Agricultural Research Journal
Year : 2019, Volume : 56, Issue : 3
First page : ( 378) Last page : ( 391)
Print ISSN : 2395-1435. Online ISSN : 2395-146X.
Article DOI : 10.5958/2395-146X.2019.00063.2

Applications of RNA Interference in insect pest management

Jindal Vikas1,*, Grover Sajjan2

1Department of Entomology, Punjab Agricultural University, Ludhiana, Punjab-141 004

2Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA-68588

*Corresponding author: vikas_ento@pau.edu

Online published on 30 September, 2019.

Abstract

RNA interference (RNAi) is a technique which is being used for analyzing the functions of individual genes by employing loss-of-function analyses. It has been widely used in entomological research to study the functions of genes in variety of insects. This technology has great potential in insect pest management due to its high specificity. In this paper, we focus on the approaches of introducing dsRNA (double strand RNA) into insect like microinjection, oral feeding, topical, plant mediated and nanoparticles. The significant effects of silencing various genes involved in morphogenesis, reproduction and detoxification of insecticides in insect pests are also discussed.

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Keywords

Delivery methods, Insects, Phenotypic effects, RNA interference.

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