Assessment of hepatoprotective consequence of Alpinia galanga extract against ethanol and carbon tetrachloride induced liver damage on experimental animal Srivastava Archita1,*, Kumari Archna2, Tripathi Abhishek Kumar1, Singh Sunil Kumar1, Srivastava Vineet1 1Department of Pharmacology, United Institute of Pharmacy, Naini, Prayagraj, 211010, U.P, India 2Department of Pharmacology, United Institute of Pharmacy, Naini, Prayagraj, 211010, U.P, India *Corresponding Author E-mail: architasrivastava2009@gmail.com
Online published on 4 May, 2024. Abstract The current study is design to evaluate the hepatoprotective action of an aqueous extract derived from the rhizome of Alpinia galanga in male Wistar rats. This assessment is conducted using a modified animal model that simulates liver toxicity induced by alcohol and carbon tetrachloride (CCl4) exposure. 30Wistar rats were divided into five groups (n=6) as the procedure was designed to last up to eight weeks. All the animals were fed with alcohol in free access, having a concentrationof 4% (v/v) ethanol, Additionally. The CCl4 is administered orally, which means it is given through the mouth. This can be done using a gavage needle or other oral administration methods. The dose of CCl4 is calculated based on the body weight of the subjects, with a dosage of 0.2ml per kilogram of body weight in ratio of 1:1.A drug Alpinia galanga aqueous extract (AEAG) was dissolved in unionized water and administered orally using an oral gauge in a single dosage at a rate of 500mg/kg body weight. In addition, the standard group received a standard medication, silymarin, at a dose of 25 mg/kg. Rats were killed at the conclusion of the trial, and a liver were taken for histological investigations and other biochemical testing, including lipid profiles, liver function tests, antioxidant activity tests. Each experimental group's body weight was checked once each week. The results indicatethe curative group was only marginally different from the preventive group based on biochemical parameters, antioxidant activity, and histological evaluation, whereas the animal treated with the preventive group is much more similar to the standard treated group than the curative group. Top Keywords Alpinia galanga, Antioxidant activity, Hepatotoxicant, Alcohol, CCl4-induced liver toxicity. Top |