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Research Journal of Engineering and Technology
Year : 2010, Volume : 1, Issue : 2
First page : ( 79) Last page : ( 81)
Print ISSN : 0976-2973.

Optimization of Amylase Production from Aspergillus oryzae MTCC 1847 by Submerged Fermentation

Yamunarani S.R.1, Kumar M. Dinesh1, Balachander B.1, Tamilarasan K.1, Muthukumaran C.2,*

1Department of Biotechnology, Madha Engineering College, Chennai -600069, India

2Department of Biotechnology, SRM University, Kattankulathur, Chennai -603203, India

*Corresponding Author E-mail: biopearl1981@gmail.com

Online published on 7 March, 2013.

Abstract

This paper mainly focused to study and optimizes the parameters like carbon sources, substrate concentration, pH, Temperature for the maximum production of amylase by Aspergillus oryzae MTCC 1847. Different Carbon sources such as Sweet potato, Rice bran and Wheat Bran were to be used as the substrate for the enzyme production. The enzyme activity was observed high when the medium contained Sweet Potato as Carbon substrate with 2% concentration, pH of 6.5, Temperature of 35°C.

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Keywords

Sweet potato, Rice Bran, Wheat Bran, Aspergillus oryzae.

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