The chloroplast genome of black currant (DNA.) cultivar ‘Kipiana’: Genome structure and chloroplast simple sequence repeats Denis Goryunov2, Anna Pikunova1,*, Sergey Knyazev3, Maria Dolzhikova1, Anna Pavlenko1, Svetlana Goryunova4 1Laboratory of Biochemical Genetics, Russian Research Institute of Fruit Crop Breeding (VNIISPK), 302530, Orel, Russia 2Department of Evolutional Biochemistry, Laboratory of Animal Genosystematic, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia, (e-mail: s.apocarpum@gmail.com) 3Department of Berry Crops Breeding, Russian Research Institute of Fruit Crop Breeding (VNIISPK), 302530, Orel, Russia, (e-mail: ksd_61@mail.ru) 4Laboratory of Plant Genetics, Russian Academy of Sciences, Vavilov Institute of General Genetics, 119333, Moscow, Russia, (e-mail: svetlana.v.goryunova@gmail.com) *(e-mail: pikunova@orel.vniispk.ru)
Online published on 25 December, 2023. Abstract The study has been conducted in year 2022-2023 at Russian Research Institute of Fruit Crop Breeding (VNIISPK), 302530 Orel, Russia with help of computational resources provided by the Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. Ribes nigrum, commonly known as black currant, is a perennial deciduous shrub having an economic importance. Though of economical importance of Ribes nigrum its complete chloroplast genome sequences is not published yet. Here, the complete chloroplast genome of black currant cultivar ‘Kipiana’ was sequenced using Illumina sequencing technology and de novo assembled for the first time. Simple sequence repeats were detected and located in the chloroplast genome. The plastome is 157,450 bp in length, with a GC content of 38.1%. The chloroplast genome is features a typical quadripartite structure, consisting of a pair of inverted repeat regions of 26,007 bp for each repeat unit, separated by a large single copy region of 87,086 bp, and a small single copy region of 18,350 bp. A total of 133 genes were annotated in the plastome, including 85 protein coding genes, 37 tRNA genes, 8 rRNA genes and 3 pseudogenes (both copies of ycf15 and one copy of ycf1 gene). Totally 66 simple sequence repeats (SSRs) were identified within the assembled Ribes nigrum plastid genome. Thus, our study provides genomic resources and valuable reference for marker development and phylogenomics in Ribes. Top Keywords Chloroplast genome, Chloroplast SSRs, Genome structure, Ribes nigrum L.. Top |