Preparation and Evaluation of Phytosome of Herbal Plant of Lawsonia inermis L for Topical Application Rajput Rudra Pratap Singh1*, Chakravarty Chinmoyi2, Bhardwaj Shiv Kumar3 1Rudra Pratap Singh Rajput, Columbia Institute of Pharmacy, Raipur 2Chinmoyi Chakravarty, Jeypore College of Pharmacy, Jeypore, Odisha, India 3Shiv Bhardwaj, Columbia Institute of Pharmacy, Raipur *rudra.p.s007@gmail.com
Online published on 22 January, 2021. Abstract Lawsonia inermis L. (Lawsone) was reported to contain carbohydrates, proteins, flavonoids and phenolic compounds, alkaloids, terpenoids. The aim of this study was to prepare and evaluate the phytosome containing lawsone. The phytosomes (P1 - P4) containing different molar ratios (1:1, 1:2, 2:1 and 2:2) of lawsone and soya lecithin were prepared by the antisolvent precipitation technique. The phytosomes were evaluated for % yield, particle size analysis, % EE and characterized by FTIR, DSC and SEM. Antifungal activity of phytosome of lawsone was evaluated on Candida albicans (NCIM 3471) fungi by using ketoconazole as standard drug. The in-vitro permeation study was carried out on rat skin. The anti-inflammatory activity was evaluated in male wistar rats. The phytosomes of lawsone P1 and P2 showed better % yield, drug content, particle size and entrapment efficiency as compared to other phytosomes P3 and P4. The infra-red (FT-IR) and Differential Scanning Calorimetry (DSC) studies of phytosome of lawsone revealed that there was no interaction between the plant drug and phospholipids. SEM data showed that phytosome of lawsone P1 has irregular size vesicles consisting of soya lecithin and it was found to be intercalated in the lipid layer. Antifungal activity of phytosome P1 (1:1) showed the better zone of inhibition as compared to phytosome P2 (1:2), drug lawsone and standard drug ketoconazole after 3 days. In-vitro permeation study of phytosome gel of lawsone (PG) through excised rat skin showed 92.91% of drug permeation up to 6 h. The anti-inflammatory activity of gel of phytosome of lawsone showed significant anti-inflammatory activity as compared to gel of drug lawsone at 4 h (P < 0.001). Top Keywords Phytosome, Lawsone, Soya lecithin, Anti-solvent precipitation technique, In vitro study. Top |