High Throughput LC-MS/MSMethod for the Quantitation of Efavirenz in Human Plasma by Solid Phase Extraction using 96 Well Plate Format Rani Lade Jyothi1,*, Sangeetha J. R.1, Tangudu V. Valli Kumari1, Rao Nagabhusana2 1Mallareddy Institute of Pharmaceutical Sciences, Dhulapally, Secunderabad, India 2 India College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India *E-Mail: ladejyothirani@gmail.com
Online published on 27 September, 2019. Abstract High through put Liquid chromatography massspect rometry method has been developed and validated for the quantification of Efavirenzin human plasmausing Atazana virasan internal standard (ISTD). Following solid phase extraction (SPE) in 96 well plate format, the analyte and ISTD were run on ACE C18 4.6 ×75 mm (5.0 μm) column using an isocratic mobile phase consisting of Methanol: 0.1% formic acid in water (90: 10, v/v). The precursor and productions of the drugs were monitore donatripl equadrupole instrument operate din the positiveionization mode. The method was valid at edovera concentration range of4.999 to 5095.155ng/mL with relative recoveries ranging from 72.5 to 76.4%. The inter batch precision (%CV) across three validation runs was ≤ 5.8%. The Inter batch percent nominal determined at five QC levels (LLOQ, LQC, MQC II, MQC and HQC) was between 98.1 to 101.0%. According to the validated results, the proposed method was found to be specific, accurate, precise and high throughput method and could be used for the estimation of Efavirenz in human plasma and can be applied for the routine analysis. Top Keywords Efavirenz, Atazanavir, Solid Phase Extraction and 96 well plates. Top |