Simultaneous Estimation of Methylcobalamine and Citicoline in its bulk and Pharmaceutical dosage form by using RP-HPLC method Kumar M. Sathish*, Pandiyan P. Shanmuga, Saibaba S.V. Research Scholar, Department of Pharmacy, Mewar University, Rajasthan *E-Mail: meruvasathish84@gmail.com
Online published on 27 September, 2019. Abstract -In the present work a simple, sensitive and specific method, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) have been developed and validated. To create a cheap, best and easy method than the previous one. ACE C8, column having 250mm x 4.6mm, 5μm in isocratic mode, with mobile phase containing Methanol: Water [40: 60, v/v] is used. The flow rate is 0.6 ml/min and effluents are monitored at 220 nm. Chromatogram showed peak at a retention time of 3.096 ± 0.008 min for Citicoline and 4.110 ± 0.008 min for Methylcobalamine. The method is validated for system suitability, linearity, precision, accuracy specificity, ruggedness, robustness, LOD and LOQ. Recovery of Citicoline and Methylcobalamine is found to be in the range of 99.22–100.11%. The LOD and LOQ for estimation of Citicoline and Methylcobalamine are found to be 0.2 μg/ml, 0.76 μg/ml, and 0.1664μg/ml, 0.5632 μg/ml respectively. Proposed method can be successfully applied for the quantitative determination of Citicoline and Methylcobalamine in Bulk drug and Pharmaceutical dosage form. Top Keywords Citicoline, Methylcobalamine, RP-HPLC, ACE. Top |