Factors governing detection of spermatozoa and choline in seminal stains Tirpude B. H., Reader, Naik R.S., Prof. & Head, Anjankar A. J., Ex Lecturer, Bhati Alok, Demonstrator, Bangal R. S., Ex. Demonstrator Dept. of Forensic Medicine & Toxicology, Mahatma Ghandhi Institute of Medical Sciences, Savagram. Dist. Wardha (M.S.) 442 102. Abstract The present study aims at finding the maximum duration for which the spermatozoa can be identified in the seminal stains on different fabrics subjected to various environmental conditions and climate. Detection of choline in the seminal stains by Thin Layer Chromatography as an alternate biochemical test for semen when chemical identification is required in absence of spermatozoa, on different fabrics subjected to various environmental conditions and climates. The complete spermatozoa without any degenerative changes could be detected for a maximum period of 130 days in cold climate and closed environment in cotton fabrics, whereas sperm showing degenerative change, but identifiable, could be found for a maximum period of 150 days in cold climate and closed environment in cotton fabrics. Choline was detectable in seminal stains for a maximum period of290 days in closed environment in khadi and cotton fabrics in cold climate. Top Key Words Semen, spermatozoa, Choline, Thin Layer Chromatography. Top |