Occurrence of Non-tuberculous Mycobacteria (NTM) in environmental samples and their characterization Maurya Vandana1, Singh Kanchan1, Gangwar Ritu1, Verma Rishendra1,*, Maurya J.N.2 1Professor of Eminence, Department of Microbiology, Mahatma Gandhi Jyotiphule Rohilkhand University, Bareilly (U.P.) 2Head of Department, Department of Microbiology, Mahatma Gandhi Jyotiphule Rohilkhand University, Bareilly (U.P.) *Correspondence: rishendra_verma@yahoo.com
Online published on 4 December, 2023. Abstract The present study was conducted to determine the occurrence of non tuberculous mycobacteria (NTM) in environmental (soil and water) samples. In all 35 samples consisting of 25 soil and 15 water from different environmental sites were investigated for NTM. 15 isolates conformed biochemically as NTM. 12 NTM isolates were subjected to PCR using allele specific primers. By PCR, 8M. fortuitum from soil and out of 2 waters samples, 1 was M. scrofulaceum and 1 M. fortuitum and two were characterized only biochemically were M. fortuitum. Conventions tests included, colony Morphology, rate of growth, pigmentation, photoreactivity, nitrate Reduction, urease and catalase. Top Keywords Enviornmental samples, NTM. Top |