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International Journal for Research in Applied Sciences and Biotechnology(IJRASB)
Year : 2020, Volume : 7, Issue : 6
First page : ( 9) Last page : ( 11)
Online ISSN : 2349-8889.
Article DOI : 10.31033/ijrasb.7.6.2

Transferring of hybrid f1 of spring wheat into dihaploid basis using andgrogen thechnologies

Wasim Aqarahim*

Junior Teaching Assistant, Department of Agronomy, Faryab University, Afghanistan

*Corresponding Author: aqarahimwasim@gmail.com

Online published on 3 February, 2021.

Abstract

Anther culture is the most technologically advanced method of obtaining doubled haploids. Dihaploid lines are required to speed up the breeding process. From 7 hybrids of first-generation (F1) of spring wheat, 15 dihaploid culture lines were obtained by anther culture. An average of 23.7 embryo-like structures was obtained on 150 anthers. The percentage of plant regeneration was 49.1%, of which there were 39.9% albino plants and 9.1% green plants. The percentage of spontaneous chromosome doubling among the obtained green plants was 35.7.

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Keywords

Anthogenesis, Anther Culture, Embryogenesis, Regeneration.

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