The effect of natural and petroleum based materials on the growth rate and antibiotic sensitivity of Pseudomonas aeruginosa Yahya Esam Bashir1*, Abdulsamad Muhanad Abdullah2, Allaq Abdulmutalib Alabeed3, Abdoallah Takwa4, Ermese Ebtehal5 1Senior Lecturer, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten, 00218, Libya 2Senior Lecturer, Department of Molecular Biology, Faculty of Science, Sabratha University, 00218, Sabratha, Libya 3Master student, Faculty of Applied Science, Universiti Teknologi MARA, Shah Alam40450, Malaysia 4Student, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya 5Student, Department of Microbiology, Faculty of Science, Al-Asmarya Islamic University, Zliten00218, Libya * Corresponding Author: essam912013@gmail.com
Online published on 4 January, 2021. Abstract Most of bacteria spend their entire lives buffeted by changing environmental conditions, depending on the surrounding circumstances, to adapt and survive these changeable conditions. Bacteria have global response systems that result in sweeping changes in gene expression and cellular metabolism. In this study, caffeine, nicotine, and petroleum based materials were used as stress factors to Pseudomonas aeruginosa, to evaluate their potential ability to induce growth rate and antibiotic resistance mutations. Bacterial cells exposed to diesel showed significant increase in growth rate, compared to the cells that exposed to the other materials. The exposure to high concentration of caffeine surprisingly tern the bacteria to become fully resistant to all tested antibiotics. Our data suggest the ability of our daily used materials to induce growth rate and antibiotic resistance mutations. Top Keywords Mutagenic, Exposure, Bacteria, Antibiotic resistance, Growth rate. Top |