Analgesic activity of Terminalia bellerica fruit pulp aqueous extract by using acetic acid induced writhing test in Swiss albino mice by peripheral mechanism Kumar D Barath1,*, Chandrashekar R.2, Manohar V. R.3, Rai Mohan Das4 1Tutor, Dept. of Pharmacology, A.J. Institute of Medical Sciences and Research Centre, Mangalore, Karnataka, India 2Assistant Professor, Dept. of Pharmacology, A.J. Institute of Medical Sciences and Research Centre, Mangalore, Karnataka, India 3Professor, Dept. of Pharmacology, A.J. Institute of Medical Sciences and Research Centre, Mangalore, Karnataka, India 4Professor & HOD, Dept. of Pharmacology, A.J. Institute of Medical Sciences and Research Centre, Mangalore, Karnataka, India *Corresponding Author: Email: drbharathmys@gmail.com
Online published on 3 January, 2019. Abstract Objective To evaluate analgesic activity of Terminalia bellerica fruit pulp aqueous extract by acetic acid induced writhing reflex test in Swiss albino mice by peripheral mechanism. Materials and Methods Albino mice of Swiss strain (Male/Female-equal number), were `selected for the study and each mouse weighed about 15-30gms. Five experimental groups made assigned as Control, Standard and three groups of test drugs at different doses, and the doses were Gum acacia (1%), 10ml/kg, Diclofenac sodium (30mg/kg), Terminalia bellerica 9, 18 and 36 mg/kg respectively. All the drugs were administered orally one hour before commencing the experiment followed by injecting 0.1 ml of 0.6% acetic acid intraperitoneally and writhing reflex was observed for next five minutes. Observations recorded as mean ± SD. Results obtained were analyzed by using ANOVA (one-way) statistical test followed by Dunnet's multiple comparison test and if p value <0.05 was considered statistically significant. Results Our study reveals that aqueous extract of Terminalia bellerica fruit pulp in the dose of 9mg/kg (4.83±1.47=p<0.045), 18mg/kg (4.33±2.42=p<0.024) and 36mg/kg (4.17±2.40=p<0.019) significantly decreased the total number of writhing reflex at the doses (9, 18 & 36 mg/kg) by acetic acid induced writhing reflex test in Swiss Albino mice when compared to control. Conclusion Aqueous extract of Terminalia bellerica fruit pulp exhibited peripheral action in all the dose of 9, 18 and 36 mg/kg by acetic acid induced writhing reflex test in Swiss albino mice. Top Keywords Aqueous extract, Fruit pulp, Peripheral analgesic activity, Terminalia bellerica, Writhing test. Top |