Evaluation of anticancer activity of Melaleuka Alternifolia. (i. e. tea tree oil) on Breast cancer cell line (MDA MB)-An invitro study Byahatti Sujata1,*, Bogar Chetana2, Bhat Kishore3, Dandagi Girish4 1Professor, Dept. of Oral Medicine and Radiology, M M's N.G. Halgekar Institute of Dental Sciences and Research Centre, Rajiv Gandhi University Belgaum, Karnataka 2Research Officer, Dept. of Microbiology and Immunology, M M's N.G. Halgekar Institute of Dental Sciences and Research Centre, Rajiv Gandhi University Belgaum, Karnataka 3Professor and HOD, Dept. of Microbiology and Immunology, M M's N.G. Halgekar Institute of Dental Sciences and Research Centre, Rajiv Gandhi University Belgaum, Karnataka 4Professor and HOD, Dept. of Pulmonary Medicine, Belgaum Institute of Medical Sciences, Belagavi, Karnataka, India *Corresponding Author: Email: sujatabyahatti78@gmail.com
Online published on 14 January, 2019. Abstract Introduction and Aims According to Globocan 2012, India along with United States and China collectively accounts for almost one third of the global breast cancer burden. India is facing challenging situation due to 11.54% increase in incidence and 13.82% increase in mortality due to breast cancer during 2008–2012. The current treatment modality has issues of drug resistance and side effects. Many anticancer drugs currently used clinically have been isolated from plant species hence, investigation of plant species as a source of experimental therapeutic agents, in treating cancer is currently gaining a lot of importance. One such naturally available plant extract Melaleuka Alternifolia (TTO) which belongs to the family of essential oils is a very good antibacterial, antifungal, antiviral, antiprotozoal and anti-inflammatory agent. But currently there is a lot of importance has been given for its anticancer effect. Hence our aim is to evaluate anticancer activity of Melaleuka Alternifolia on Breast cancer cell line (MDA MB) by MTT assay an invitro method. Material and Methods Before the start of the study ethical clearance was obtained from Institutional Review Board. The cytotoxicity checked for Breast cancer cell line (MDA MB) and NIH 3T3 mouse fibroblasts which was used as a control in our current study. These cell lines were procured from National Centre for Cell Science (NCCS) Pune, India. 1. MTT solution preparation (stock solution): 5 mg in 1 ml of PBS. 2. Cell culture: The cell lines were maintained in 96 wells micro titer plate containing MEM media supplemented with 10% heat inactivated fetal calf serum (FCS), containing 5% of mixture of Gentamicin (10ug), Penicillin (100 Units/ml) and Streptomycin (100μg/ml) in presence of 5% CO2 at 37ºC for 48–72 hours. 3. Cytotoxicity Assay: In vitro growth inhibition effect of test compound was assessed by calorimetric or spectrophotometric determination of conversion of MTT into Formazan blue by living cells. Results The results represent the mean of five readings. The IC50 value of tea tree oil for breast cancer cell line (MDA MB) after 48 hrs was 25μg/ml. Spearmans rho's Correlation showed P value <0.05 indicating there was statistical significant results obtained when TTO was treated with breast cancer cell line (MDA MB) for 48 hrs incubation period. Conclusion TTO has a promising anticancer property against Breast cancer cell line (MDA MB) with its IC50 value 25μg/ml. Hence this TTO with its greater efficacy related to its anticancer activity can be brought to the level of clinical trials in the coming future. Top Keywords Cytotoxicity, MTT assay, MDAMB cell line, Tea tree oil. Top |