A modified DNA isolation protocol for high-quality DNA and long-term storability in grasspea (Lathyrus sativus L.) Ramya K. R., Gupta R.1, Pandey A.1, Tripathi K.1, Bhatt K. C.1, Singh R.1,* Division of Plant Genetic Resources, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India 1ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India *Corresponding Author: R. Singh, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India, E-Mail: rakesh.singh2@icar.gov.in
Online Published on 01 February, 2024. Abstract Grasspeas pose challenges in obtaining high-quality DNA due to their seed and leaves’ high protein, phenols, secondary metabolites, and neurotoxins. This study investigated the challenges faced by the CTAB method in DNA isolation, subsequent enhancements in quality and storage with a modified CTAB approach using triton, PVP, high salt TE and sodium acetate to extract the high-quality genomic DNA from grasspea. Top Keywords Grasspea, CTAB, Protein, PVP, Triton. Top |