Study on Highly Efficient Direct Shoot and Root Organogenesis from Cotyledon and In vitro Derived Root and Leaf of Citrus jambhiri Lush. Sharma Priyanka*, Roy Bidhan1 Seed Science and Technology Programme, Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat-785 013, India 1Department of Seed Science and Technology, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar-736 165, India *E-mail: sprianca133@gmail.com
Online Published on 20 February, 2024. Abstract A protocol was developed for the direct regeneration of plantlets from C. jambhiri leaf explants that had undergone in vitro regeneration. The goal of the current investigation was to determine how well C. jambhiri seed cotyledons might be directly used to regenerate shoots. The cotyledons enlarged as the seeds initiated its germination. In MS medium supplemented with casein hydrolysate, the highest response to cotyledon expansion was observed . The number of enlarged cotyledons cultured by the medium treated with 50 mg L-1 of casein hydrolysate was highest (96%) and was followed by 100 mg L-1 (84%) and 200 mg L-1 of casein hydrolysate (78%). On the synthetic medium supplemented with 50 mg L-1 of casein hydrolysate (1.628 g/cotyledon), 100 mg L-1 of casein hydrolysate (1.148 g/cotyledon), and 200 mg L-1 of casein hydrolysate (1.050 g/cotyledon), the largest cotyledons by weight were produced. When the seeds were inoculated on MS media supplemented with 1.0 mg L-1 of IAA and 1.0 mg L-1 of IBA, direct regeneration from the roots of germinating seeds was observed in C. jambhiri. On MS media supplemented with various combinations and quantities of plant growth regulators, leaves excised from axenic shoot cultures were utilised to stimulate organogenesis. Adding 0.50 mg L-1 of dicamba to the medium indicated minimum percentage of leaf callus. As the leaf gradually dried up, the callus began to regenerate on the callus induction medium. Depending on the particular Citrus species, the quality of the germplasm, and the tissue culture practitioner’s experience, direct organogenesis results might vary. To obtain promising results, factors including sterilisation, culture medium composition, and ambient factors should be properly optimised. Top Keywords Direct regeneration, PGR’s, Enlarged cotyledons, Casein hydrolysate, Conservation. Top |