Biochemical Mechanism of Insecticide Resistance in Spodoptera litura (F) Populations from Uttarakhand Joshi Rashmi1,*, Gaur Neeta1, Mathpal Sudha1 1Department of Entomology, College of Agriculture, Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, US Nagar263145, Uttarakhand, India *Email: rashmipandey.hdw@gmail.com (corresponding author): ORCID ID 0000-0003-2343-1463
Online Published on 04 January, 2024. Abstract Spodoptera litura populations were collected from different lowland areas and doon valley situated in Uttarakhand and insecticide resistance effect was studied for indoxacarb 14.5% SC, chlorantraniliprole 18.5%SC, fipronil 5% SC, chlorpyriphos 50%+cypermethrin 5% EC and profenofos 40%+cypermethrin 4% EC, also the specific activity of carboxylesterase, acetyl choline esterase, mono-oxygenase and glutathione-s-transferase was studied for biochemical analysis of detoxification enzymes. The scale of infestation was measured and related to temperature and humidity. High infestation has been observed in areas with optimum temperature and humidity. Highest resistance in the study was found against profenofos 40%+chlorpyriphos 4% and fipronil 5%SC with LC50 243.63 μg/ml and 214.22 μg/ ml respectively. On biochemical analysis of detoxification enzymes, it was observed that the highest activity of all the enzymes was observed in Mota Haldu population. Hence, overproduction of detoxification enzymes was found to be responsible for insecticide resistance in S. litura populations studied. Top Keywords Spodoptera litura, Carboxylesterase, Acetyl choline esterase, Mono-oxygenase, Glutathione-S-transferase, Uttarakhand, Insecticide resistance, Himalayan lowlands, LC50, Detoxification enzymes. Top |