Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2004, Volume : 25, Issue : 1
First page : ( 15) Last page : ( 18)
Print ISSN : 0790-9320.

Application of RBPT, SAT and Avidin-Biotin Serum elisa for detecting brucellosis among livestock in Himachal Pradesh

 Charanjeet, Sharma Mandeep*, Katoch R.C., Dhar Prasenjeet, Kumar Rajinder

Department of Veterinary Microbiology, College of Veterinary Science, CSK, Himachal Pradesh Krishi Vishvavidyalya, Palampur - 176 062 (H.P.)

*Correspondong author

A total of 1113 serum samples from cattle, buffaloes, sheep and goats were collected from different parts of Himachal Pradesh and subjected to detection of antibodies against brucellae. The sera samples were examined by RBPT, SAT and avidin-biotin serum ELISA tests. Out of 641 cattle, 146 buffaloes, 141 sheep and 185 goats’ serum samples tested, only 17 cattle, 5 buffaloes, 22 sheep and 10 goats were found positive, respectively. The results were based on RBPT as this test is considered suitable and usually recommended for random screening of animals against brucellosis as per OIE. The seroprevalence of brucellosis was uppermost in sheep and low down in cattle irrespective of their age. It was also high in females having history of reproductive problems.

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