Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2003, Volume : 24, Issue : 2
First page : ( 181) Last page : ( 185)
Print ISSN : 0970-9320.

Induction of mucosal immunity in poultry after oral immunization with a single dose of microencapsulated eds-76 virus

Dandapat S.*, Banerjee D., Rajan, Kataria J.M., Ram G.C.¹, Yadav M.P.

Director's Laboratory Indian Veterinary Research Institute, Izatnagar-243 122 (U.P.)

*Corresponding author

¹Immunology Section

Received:  26  January,  2004.

Abstract

Formalin inactivated EDS-76 virus was encapsulated in the poly-lactide-co-glycolide (PLG) microspheres by solvent evaporation technique. White Leghorn layer chicks were orally immunized with a single dose of 75 µg of the microencapsulated EDS-76 virus and the immune responses induced at mucosal sites were evaluated. In the intestinal and tracheal washings of the immunized chicks, considerably higher levels of virus specific IgA were detected with the peak titres of 30 and 24, respectively at 3 wk PI as compared to the conventional inactivated EDS-76 vaccine which showed very negligible IgA level (titre <2.0). The cell-mediated immune responses induced at mucosal sites were evaluated by assessing the lymphoproliferative and cytotoxic activities of the intestinal intraepithelial lymphocytes (iIEL). It was found that the lymphoproliferative responses induced by microencapsulated EDS-76 virus were significantly higher (p<0.05) with a peak stimulation index value (1.314 ± 0.022) at 3rd wk PI as compared to the microsphere alone (control) and the conventional EDS-76 vaccine. The cytotoxic activities were also induced by the iIEL after oral delivery of microspheres containing the EDS-76 virus.

Top