Study of Changes in Biochemical Parameters Due to Hyperglycemia in Cases of Diabetes Mellitus Kumar Amresh1, Prasad Shiv Shankar2,*, Gunjan3, Sinha Rajiv Ranjan4 1Senior Resident, Dept. of Biochemistry, Govt. Medical College, Bettiah 2Senior Resident, Dept. of Medicine, Patna Medical College, Patna 3Specialist Medical Officer, Obstetrics and Gynaecology, Bihar Sharif 4Consultant (DM Gastroenterologist), Curis Hospital, Patna *Corresponding author: Dr. Shiv Shankar Prasad, Senior Resident, Department of Medicine, Patna Medical College, Patna, Email- dramreshkmch@gmail.com, Mobile- +919234001202
Online published on 6 April, 2019. Abstract Objective Diabetes Mellitus (DM) is the most common endocrinological disorder which is characterized by metabolic abnormalities and long-term complications. The purpose of this study was to investigate the role of hyperglycemia on various parameters like Malondialdehyde (MDA), a parameter to study increased oxidative stress, as hyperglycemia leads to increased lipid peroxidation. Adenosine Deaminase (ADA) activity which is suggested to be an important enzyme for modulating the bioactivity of insulin and Free Fatty acids (FFA) to study dyslipidemias which are associated with diabetes. Materials and Method MDA, ADA and FFA levels in serum were measured spectrophotometrically in 100 patients of type 2 Diabetes Mellitus (DM) and also in 50 healthy controls. The patients were divided into three groups according to the levels of Glycosylated hemoglobin (HbA1c); Group I (with HbA1c 6–10%), Group II (with HbA1c 10–13%) and Group III (with HbA1c >13%). The results were also compared with the control group which had HbA1c of 4–6%. Results All the three parameters, ADA, MDA and FFA levels were found to be significantly higher in the case subjects as compared to the controls (p<0.001). In addition to this correlation, the study revealed that MDA levels are positively associated with FBS and PPBS and also, that ADA is positively correlated with MDA (r= +0.57, p<0.001). Conclusions These findings suggest that hyperglycemia is associated with increased levels of ADA and that it is one of the factors which lead to the increased production of oxidative stress and also the derangements of lipid metabolism which are associated with DM. Also, ADA has got a role in increasing lipid peroxidation by reactive oxygen species (ROS) generation, as reflected by increasing MDA levels. Top Keywords Diabetes Mellitus, Glycosylated Hemoglobin, Adenosine Deaminase, Malondialdehyde, Free Fatty Acids. Top |