Effect of cystein hydrochloride and Vitamin E supplementation on quality of cryopreserved hariana bull semen Pal Ajendar Singh, Singh Vijay*, Patel Akhil Department of Veterinary Gynaecology& Obstetrics, College of Veterinary Science & Animal Husbandry, Uttar Pradesh Pandit Deendayal Upadhyaya Pashuchikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura-281001, U.P. *Corresponding author's email ID: vijaysohlot@yahoo.co.in
Online published on 27 May, 2021. Abstract The present study was aimed to evaluate the beneficial effects of supplementation of cystein hydrochloride and Vitamin E on seminal attributes during different stages of semen cryopreservation in Hariana bull. Thirty two ejaculates from four Hariana bulls were collected after initial examination the qualified ejaculates were divided into three aliquots; one aliquot was extended with glycerolated egg yolk tris citrate (GEYT) extender (Control), second aliquot was extended with GEYT supplemented with Cystein hydrochloride @ 5.0 mM (T1) and the third aliquot was extended with GEYT supplemented with VitaminE @ 2.5 mM (T2). All semen samples were equilibrated and cryopreserved. The effect of cystein hydrochloride and vitamin E was determined by assessment of sperm viability, progressive motility, hypo-osmotic response, and acrosomal integrity at three stages i.e. after dilution, pre-freeze and post-thaw. It was observed thatsupplementation of cysteine hydrochloride (5mM) and vitamin-E (2.5 mM) caused significant (P<0.05) increase in all sperm attributes at post thaw semen. Top Keywords Bull, Cryopreservation, Cystein hydrochloride, Vitamin c, Semen. Top |