Identification of an effective genotyping assay for marker assisted selection of high oleic acid content trait in groundnut (Arachis hypogea L.) Mythili S. R.1, Manivannan N.1,*, Kalaimagal T.2, Paranidharan V.3, Janaki P.4 1Centre of Excellence in Molecular Breeding, Tamil Nadu Agricultural University, Coimbatore - 641003, India 2Department of Oilseeds, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore - 641003, India 3Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore - 641003, India 4Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Coimbatore - 641003, India *E-Mail: nm68@tnau.ac.in
Online Published on 22 January, 2024. Abstract The substitution and insertion mutation in the fatty acid desaturase gene is responsible for increased oleic acid in groundnut. It can be identified by various genotyping assays with allele specific primers. Present study compares the efficiency of three earlier reported genotyping assays to identify the effective and accurate method. A high yielding groundnut variety GJG 33 was crossed with high oleic acid content variety Girnar 4. Both parents and 23 BC2F2 plants were used for genotyping. Allele specific primers assay proposed by Chen et al. (2010), the CAPS marker assay proposed by Chu et al. (2007 and 2009) and the primers assay proposed by Yu et al. (2013) were used for comparisons. The primer pairs reported by Yu et al. (2013) was found to be efficient in terms of time and cost. Hence these primers may be recommended for utilization in marker assisted breeding to evolve high oleic acid content groundnut genotypes. Top Keywords Groundnut, High oleic acid content, Mutant allele, Wild allele, Marker assisted selection. Top |