Assessment of In vitro regeneration ability of mango genotypes Pandey Kuldeep1,4,*, Kishor Arun1, Singh Ankit1, Singh Sanjay Kumar1, Sreevathsa Rohini2, Vinod3, Srivastav Manish1,** 1Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India 2ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi-110 012, India 3Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India 4Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229 (Uttar Pradesh), India *Kuldeep Pandeypandeykuldeephort@gmail.com
**Manish Srivastav, (Corresponding authors) mns_fht@rediffmail.com
Online Published on 18 March, 2023. Abstract Present investigation was carried out with the objectives to assess the in vitro regeneration potential of mango through in ovulo embryo and shoot tip culture. It was postulated from the present that mango cv. Amrapali exhibited maximum embryo growth during in ovulo embryo maturation. However, Kerala-1 showed least number of embryos showing growth during in vitro maturation. Amrapali also showed maximum germination (66.66%) of embryos, whereas cultivar Kerala- 1 had the minimum germination (38.95%) of matured in ovulo embryos. The days taken for emergence of radicle ranged from 23.5 (Kurukkan) to 33.1 (Olour), and period required for emergence of plumule ranged from 28.0 (Kurukkan) to 34.1 (Olour). in vitro and ex vitro survival was maximum in Amrapali (55.5 %). Survival of shoot tip explants from mango genotypes during in vitro culture revealed non-significant difference during first 15 days of culture. However, significant result were obtained at 20 DAC, in which Amrapali genotype showed maximum survival (24.89%), followed by Pusa Arunima (24.21%) and Kerala-1 had the minimum survival (18.79%). It was also evident that necrosis of shoot tip explant during in vitro culture was significantly high. The maximum necrosis of explants was in Olour (21.44%) at 5 days after culture, Kurukkan (25.00% and 34.98%) at 10 DAC and 15 DAC, respectively. Amrapali had the maximum necrosis (46.55%) at 20 days after culture. Shoot tip cultures also suffered from microbial contamination. Maximum microbial contamination found in the Kerala-1 (10.50, 15.44, 19.77 and 39.79%) at 5, 10, 15 and 20 days after culture, respectively. Minimum microbial contamination was found in Kurukkan (6.03, 8.19% and 14.58%) at 5, 10 and 15 DAC, respectively. However, 20 days after culture contamination was comparatively more (34.98%). Amrapali had the lowest percent of contamination (28.36%) at 20 DAC compare to other mango genotypes. From the present study, it is concluded that in vitro embryo culture is better strategy for regeneration of mango compared to shoot tip culture. Top Keywords Genotypes, In vitro regeneration, Mangifera indica, Mango, Shoot tip culture. Top |