Heavy metals stress stimulation of Escherichia coli nissle 1917 –Proteomic analysis by using two-dimensional polyacrylamide gel electrophoresis (2D page) Kumari J. Pramoda*, Seshapani P. Dept.of Microbiology, S.V.U PG Centre, Kavali-524201, Andhra Pradesh, India *Corresponding author: Dr. J Pramoda kumari, Assistant Professor, Dept. of Microbiology, S.V. UPG Centre, Kavali -524207, AP, India Email: Pramodakumari@gmail.com, Mobile: +919491166222
Online published on 26 February, 2013. Abstract Heavy metals bound to proteins play a key role in structure stabilization, catalysis, and metal transport in cells, can be at high concentration toxic to all branches of life including microbes, forming complex compound's with in the cells The nature of the stress experienced by Probiotic Escherichia coli Nissle 1917 exposed to study for it's tolerance to heavy metals i.e. Cadmium and mercury by growing in Luria-Bertani broth diluted 1:50 in Dulbecco's modified eagle's medium (DMEM) and are incubated for 9 and 6 h respectively, at 37°C in a 5% (v/v) CO2 atmosphere. Proteomic approach involving two-dimensional polyacrylamide gel electrophoresis (2D PAGE) was used to identify the differentially expressed proteins, when E.coli Nissle 1917 exposed to heavy metal stress. Analysis of the differentially expressed proteins as well as the newly synthesized proteins along with the results obtained growth and protein profiles indicating the involvement of all these factors in the survival of this organism in the presence of heavy metals. Top Keywords E.coli Nissle 1917, Heavy Metals, 2DGel Electrophoresis. Top |