In vitro feed fermentation with the rumen liquor of buffaloes fed on diet supplemented with phyto-feed additives Soni P.K., Chaudhary L.C.*, Kala Anju, Rahman H.1, Agarwal Payal Rumen Microbiology Laboratory, CAFT Animal Nutrition ICAR-IVRI, Izatnagar-243122, India 1ILRI, New Delhi *Corresponding author: lcchaudhary1@rediffmail.com
Online published on 1 October, 2022. Abstract A study on in vitro feed fermentation with the rumen liquor of the buffaloes (12–15 months of age with average body weight 164±4.0kg) supplemented phyto-feed additives (FAs) was conducted to evaluate the long term feeding of the additives for its sustainability of inhibiting methane production. Monthly (for 6 months) in vitro experiments were performed using the rumen liquor of buffalo calves fed on diet supplemented with no additive (T0 control), with FA I (blend of garlic bulb, ajwain seed, harad seed pulp and soapnut berries mixed in 1:1:1:1 ratio) @ 1% of DMI (T1), with FA II (ajwain oil) @ 1ml of per kg dry matter intake (T2) and with FA I and FA II switched alternatively after every 15 days (T3). There was reduction (P<0.05) in total gas and methane production irrespective of the period and this inhibition was maximum (22.2 and 46.56 percent) with the rumen liquor of T3 group. There were no effects of feed additives on in vitro true digestibility, total volatile fatty acids and molar proportion of acetate, propionate, butyrate, A:P ratio and ammonia-N at any period. However, significant increase was noticed in partitioning factor in T3 treatment. Alternate supplementation of the two feed additives had no additional advantage. Further, the results revealed that the feed additives did not lose their anti-methanogenic characteristic as there was methane inhibition till the end of the experiment. Top Keywords Ajwain oil, Buffalo calves, Garlic, Harad, Methane, Phyto-feed additives, Soapnut. Top |