Effects of facultative heterofermentative lab, enzymes and fermentable substrates on the fermentation quality, aerobic stability and In vitro ruminal fermentation of rice straw Chen Y.F., Ouyang J.L., Shahzad K.1, Qi R.X., Wang M.Z.* College of Animal and TechnologyYangzhou UniversityYangzhou-225009, China 1Department of Biosciences, COMSATS University Islamabad, Park Road, Islamabad-45550, Pakistan *Corresponding author: mengzhiwangyz@126.com
Online Published on 21 July, 2022. Abstract Chen, Y.F., Ouyang, J.L., Shahzad, K., Qi, R.X. and Wang, M.Z. 2022. Effects of facultative heterofermentative LAB, enzymes and fermentable substrates on the fermentation quality, aerobic stability and in vitro ruminal fermentation of rice straw. Animal Nutrition and Feed Technology, 22: 67–78. This study was conducted to investigate the effects of facultative heterofermentative bacteria, enzymes and fermentable substrates on quality and aerobic stability of rice straw. Dry rice straw was processed into 6 groups in 0.3 x 0.5m vacuum bags with no additives as a control (CON), Bacillus licheniformis + Aspergillus niger + glucose (BAG), B. licheniformis + cellulase + starch (BCS), Lactobacillus plantarum + Trichoderma viride + starch (LTS), L. plantarum+ cellulase + glucose (LCG) and Enterococcus faecalis + cellulase + bran (ECB). After 45 days storage, straw samples were subjected to chemical analyses for quality assessment, and the remainders were exposed to oxygen for 15 days for further aerobic stability assessment. Compared with the control, the pH value and ammonia nitrogen/total nitrogen was decreased in all treatments. The lactic acid content increased in LTS and LCG, whereas acetic acid and butyric acid contents decreased significantly (P<0.05). After the oxygen exposure, residual sugar content was higher, whereas, pH and NH3-N/TN concentration was lower in LCG straw as compared to others. In conclusion, the application of LCG is more effective for enhancing quality and aerobic stability. Top Keywords Aerobic stability, Enzymes, Fermentable substrates, Fermentative bacteria, In vitro fermentation, Lactic acid bacteria, Rice straw. Top |