Simultaneous Estimation of Metronidazole and Amoxicillin in Synthetic Mixture by Ultraviolet Spectroscopy Tiwari Gaurav1,*, Tiwari Ruchi1,, Srivastava Brijendra2, Rai Awani K1, Pathak Kamla3 1Department of Pharmaceutics, Pranveer Singh Institute of Technology, Kalpi Road, Bhauti, Kanpur-208020 (UP) India 2Department of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India 3Rajiv Academy for Pharmacy, Department of Pharmaceutics, Delhi-Mathura Bypass, Chattikara, Mathura (UP) India *Corresponding Author E-mail: tiwari_mpharm@rediffmail.com
Online published on 13 March, 2013. Abstract Two simple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of metronidazole and amoxicillin in combined dosage form have been developed. First method employs formation and solving of simultaneous equation using 319 nm and 273.8 nm as two analytical wavelengths for both drugs in phosphate buffer pH 7.4. The second method is Q value analysis based on measurement of absorptivity at 319 nm and 289 nm (as a iso-absorptive point). Metronidazole and amoxicillin at their respective λmax 319 nm and 272 nm and at iso-absorptive point 289 nm shows linearity in a concentration range of 10–50 mcg/mL. The results of analysis have been validated statistically, the standard deviation lies in the range of 0.012 -0.071 for amoxicillin and 0.028 -0.153 for amoxicillin in case of simultaneous equation method and 0.020 - 0.098 for amoxicillin and 0.032 - 0.231 for amoxicillin in case of Q - analysis method. Recovery studies range from 98.45 - 99.0% for amoxicillin and 101.10 – 102.06% for metronidazole in case of simultaneous equation method and 99.60 – 101.0% for amoxicillin and 100.20–100.90% for metronidazole in case of Q - analysis method confirming the accuracy of the proposed method. Top Keywords Amoxicillin, Metronidazole, UV spectroscopy, Simultaneous equations, Q – analysis. Top |