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Determination of Nicotine from Tobacco Hariharan P1, Sivakumar N2 1Research Scholar, Department of Chemistry, AMET University, Chennai 2Professor, Department of Chemistry, AMET University, Chennai Online published on 24 October, 2017. Abstract Another superior fluid chromatography combined with mass spectrometry technique (LC/MS/MS) for measurement of nicotine from tobacco was explained. It was used an Atlantis HILIC, 100 mm x 3.0 mm i.d., 3 μm segment with a versatile stage containing acetonitrile/arrangement 0.2% formic corrosive in water. The ionization was upgraded utilizing ESI(+) and improved selectivity was accomplished utilizing couple mass spectrometric examination. The antecedent to item particle moves of m/z 163 > (105.8, 131.8) were utilized to quantify the nicotine focuses. The evaluation was made utilizing the outside standard strategy. The adjustment bends were made on run 0.044 μg/ml. Nicotine content was resolved in 40 brands of cigarettes accessible in Romania. With our system, we got estimations of nicotine in tobacco in the vicinity of 7.5 and 17.6 mg/g. Top Keywords Nicotine, Tobacco, Smoking, Chromatographic. Top | |
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