Screening for Parental Polymorphism Using Molecular Markers in Groundnut (Arachis hypogaea L.) Prabhu R.1,*, Manivannan N.1, Mothilal A.2, Ibrahim S.M.3 1Department of Oilseeds, CPBG, TNAU, Coimbatore-641 003, Tamil Nadu, India 2Regional Research Station, TNAU, Vridhachalam-606 001, Tamil Nadu, India 3Agricultural College and Research Institute, TNAU, Madurai-625 104, Tamil Nadu, India *email: rajprabhu03@yahoo.com
Online published on 2 January, 2017. Abstract Groundnut (Arachis hypogaea L.) is a vital food and cash crop for resource poor farmers in Asia and Africa. Constrain in groundnut yield is due to two foliar fungal diseases viz., late leaf spot and rust which cause considerable loss in most areas of the world. In order to introgress foliar disease resistance genes from GPBD 4, a backcross breeding programme was initiated in groundnut and the susceptible genotypes ICGV 00350, ICGV 03128 and VRI 2 were used as recurrent parents. They were surveyed with eight foreground and 196 background SSR primers toidentify polymorphic markers that would discriminate susceptible genotypes from the resistant one. Eight foreground markers linked to the late leaf spot and rust resistant QTL viz., IPAHM103, GM2079, GM2301, GM1536, GM1573, GM1009, GM2009 and seq8D09 were surveyed and identified as polymorphic for all the genotypes against GPBD 4. In the same way, a total of 196 microsatellite loci that span across the entire groundnut genome were utilized for the identification of parental polymorphism for background analysis. Out of 126 amplified markers, 53 (42.1%) and 50 (39.7%) markers were polymorphic for the combination ICGV 00350 × GPBD 4 and ICGV 03128 × GPBD 4, respectively. Similarly, 47 out of 123 amplified primers (38.2%) were found polymorphic between the parents VRI 2 and GPBD 4. Hence, the SSR markers that are linked (foreground selection)/unlinked (background selection) to target trait but polymorphic between both donor and recurrent parent will be utilized to introgress resistant genes from donor parent with maximum recovery of recurrent genome in future marker assisted backcross breeding programme. Top Keywords Groundnut, Parental polymorphism, Foreground markers, Background markers, Marker assisted backcross breeding. Top |