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Advances in Life Sciences
Year : 2016, Volume : 5, Issue : 7
First page : ( 2871) Last page : ( 2873)
Print ISSN : 2278-3849. Online ISSN : 2278-4705.

Protein Profiles of Soybean (Glycine max L. mer) Cultivars under Drought Stress

Kachare Satish*, Tiwari Sharad, Tripathi Niraj, Thakur Vishwa Vijay

Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur-482004

*email: agriksatish@gmail.com

Online published on 15 December, 2016.

Abstract

Drought stress led to a series of alterations including biochemical changes like accumulation of osmolite and specific proteins involved in stress tolerance. The present study aimed to identify the protein profiles of 6 cultivars of local Indian soybean: JS20–95, JS9752, JS20–108, JS-95-60, JS335 and CG Soya. Water stress treatment was imposed at 65 day after sowing and compared with plantswhich were grown on normal condition as control plants. Total proteins were isolated from leaf and quantified. The results of SDS-PAGE electrophoresis showed a specific protein band with the molecular weight of 27.5kDa were found in all stressed plants except JS97–52 in addition JS2095, JS97–52 and JS335 showed specific protein band (18kDa) under stress condition.

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Keywords

Biochemical, Soybean, SDS-PAGE, Drought stress, Protein profile.

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