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Molecular Variability of Rhizoctonia bataticola Causing Root Rot of Soybean Using RAPD Marker Belkar Y.K.*, Gade R.M. Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, (M.S), 444 104 *Email: yogesh.belkar@gmail.com
Online published on 23 December, 2016. Abstract The genomic DNA of 20 isolates of Rhizoctonia bataticola isolated from fresh mycelium of fungus grown in Potato Dextrose Broth solution and subjected to amplification with RAPD markers for diversity analysis. Amplified product of RAPD were resolved by electrophoresis and photographed under gel documentation system. Randomly selected 20 decamer primer amplified 149 RAPD marker loci. Out of these 149 bands, 111 bands (74.49%) were polymorphic. Similarity coefficient values for 20 accessions of R. bataticola in RAPDs marker were 0.36–0.99. Top Keywords Glycine max, Rhizoctonia bataticola, RAPD, Variability. Top | |
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