Induced Mutagenesis through Gamma Radiation and Ethyl Methane Sulphonate in Chickpea (Cicer arietinum L.): Developmental Changes and Resistance to the Dry Root Rot Pawar Maruti*, Gupta Om Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh *Email: marutip23@gmail.com
Online published on 23 December, 2016. Abstract Induced mutagenesis through gamma radiation and ethyl methane sulphonate was tested in chickpea three varieties to improve resistance to the dry root rot in M3 generation. The 200 Gy, 0.3% EMS and 200 GY + 0.2% EMS dose was determined as the optimum dose limit causing reduction in survival in JG 63 as compared to other two varieties in M3 generation. The effects of doses ranging from 200 to 400 Gy, 0.3% to 0.4% EMS and combination were 150 GY, 0.3% EMS to 200 GY, 0.2% EMS with untreated control determined on various developmental traits. 200 Gy, 0.3% EMS and 200 GY + 0.2% EMS treatment improved plant growth while doses of over, especially 400 Gy, 0.4% EMS and 150 GY, 0.3% EMS to reduced all the analyzed parameters of growth. Gain in resistance to dry root rot and seed yield in 200 Gy, 0.3% EMS and 200 GY + 0.2% EMSmutants was demonstrated in field experiments in M3 generation. Disease rating scale among selected M3 plantsand unirradiated plants ranged from 7.4–89.4 percent. In conclusion, all rights in M3 generation developed through gamma irradiation and ethyl methane sulphonate and their combination were screened along with their respective control for resistance to dry root rot in artificial sick plot during 2015–2016. Two varieties showed highly susceptible reaction to dry root rot. Out of of seven treatments of 3 genotypes, one genotype JG 63 exhibited highly resistant reaction by 200 GY, 0.3% EMS and 200 GY + 0.2% EMS mutagenic treatment. The mutagenic treatments proved to be effective in producing morphological mutations along with improved tolerance to dry root rot. Thus variety JG 63 with resistant to tolerant reaction for dry root rot could be used in hybridization program for transferring of resistance genes into high yielding elite cultivars/producing better recombinants. Top Keywords Rhizoctonia bataticola, (Cicer arietinum L.) and (Vigna radiata L. Wilczek), irradation, resistance. Top |