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Development of a Set of Multiplex PCR Assays for DNA Barcoding of Medicinal Plants Thakur Vishwa Vijay*, Tiwari Sharad, Tripathi Niraj Biotechnology Centre, Jawaharlal Nehru Agriculture University, Jabalpur, 482004, India *email: vijaybiotech85@gmail.com
Online published on 23 December, 2016. Abstract DNA barcoding utilizes species-specific DNA sequence information for specimen identification. During the present investigation, we used Barcodetagged PCR primers for multiplex amplification. Different combinations of primers were evaluated to amplify same templates in a single reaction. Four primer combinations were successfully amplified amplicons of desired size. This will be an economical approach for DNA barcoding of medicinal plants. Top Keywords DNA barcoding, Primers, Medicinal plants, Multiplex PCR. Top | |
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