Assessment of Genetic Diversity and Hybrid Purity Test in Safflower (Carthamus tinctorius L.) Using Simple Sequence Repeats (SSRs) Patel Nirmala Bharti*, Shrivastava Rajeev, Saxena Ritu R., Soni Pratibha Department of Genetics and Plant Breeding, Indira Gandhi Agricultural University, Raipur-492102, (C.G.), India *email: nirmalabharti.patel@gmail.com
Online published on 2 January, 2017. Abstract In this study, Safflower (Carthamus tinctorius L.) F1 hybrids and their parents were studied for identification and genetic purity testing using 16 SSR primers. This study also analyzed safflower F1 hybrids and its parents (male and female) with 16 primers to identify the band pattern which could differentiate between the parents and hybrids. Out of these 16 SSR primers only 12 primers, SES 33, SES 81, SES 85, SES 86, SES 91, SES 98, SES 100, SES 104, SES 122, SES 129 SES 143 and SES 144 showed polymorphic bands between parents and Since a lot of chances are there for contamination by selfing of female parental lines and out crossing with other plants during hybrid development programs. This seriously affects the hybrid production quality. So molecular marker tools can be effectively used to find out suchcontaminations in DNA polymorphism of respective hybrids. Top Keywords Safflower, SSR markers, F1 hybrids, Genetic purity. Top |