Multidrug resistant (MDR) Vibrio alginolyticus has become a global challenge threatening both human health and food safety. It is pathogenic to marine animals and causes wound and ear infections in humans. It also causes soft-tissue infections leading to necrosis and bacteraemia that could result in septic shock in immunocompromised humans (Amalina et al. 2019; Jacobs-Slikfa et al. 2017).

In aquaculture, the hazard of infectious diseases has led to significant stock losses causing financial losses to fanners. As an loss prevention strategy, intensive aquaculture promotes the indiscriminate use of antimicrobials, thereby causing the dissemination of antimicrobial-resistant (AMR) bacteria and resistance genes in aquaculture products and the environment (Sadat et al. 2020; Kelly et al. 1991).

The Present study is an effort to assess the prevalence of V. alginolyticus prevalence and its multidrug resistance pattern in marine fishes.

The fish samples were collected from selected local market in Uttara Kannada district of Western coast in Karnataka state. The sample was dissected and collected 5g of muscle and hologenised in saline water. The supernatant is enriched in TCBS broth with 10% NaCl. Enriched samples is spred on TCBS agar with 10% NaCl. The large colonies surrounded by yellow sucrose fermenting zone were picked and subjected for colony PCR using specific primers.

About 80% fishes were positive for V. vulnifus suggesting higher rate of prevalence; alarming the urgent need of attention for antimicrobial resistance studies and development of its control measures.

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