Hardening and acclimatization of an endandgered forest tree Givotia Rottleriformis Grif. Rambabu M.1*, Ujjwala D.2, Ramaswamy N.3 1.Department of Botany, Kakatiya Govt. College, Hanamkonda, Warangal, (T.S.) 2Department of Botany, TSWRDCW, Kothagudem, Bhadradri Kothgudem, (T.S.) 3Department of Biotechnology, Kakatiya University, Warangal, (T.S.) *Email: rambabudrbot@gmail.com
Online published on 5 October, 2021. Abstract Givotia rottleriformis is an economically important forest tree and especially used in toys making industry. The acclimatization of the in vitro developed plants is a crucial stage to make them survive under the ex-vitro conditions Hardening is a method in which the tissue culture plants developed in artificial media are habituated to grow in natural environment. Afterwards the plants are transferred to polybags filled with potting mixture and grown under shaded house for 6–8 weeks. In the present investigation, hardening and acclimatization experiments were carried out both on in vitro and ex vitro rooted plantlets. The potted mixtures containing red soil + sieved sand + vermicompost (1: 1: 1) showed good survival for in vitro and ex vitro rooted plants. Maximum percentage of survival rate was observed in the plantlets developed from ex vitro rooting. Top Key words Givotia rottleriformis, In vitro, Ex vitro, Hardening, Acclimatization. Top |