Anti-diabetic, antioxidant and toxicity studies of Grewia abutilifolia leaves extracts Rani Dolly1, Kharkwal Harsha2*, Kulkarni G.T.3, Rai Nitin4, Grover Parul5, Chander Subhash6 1Amity Institute of Pharmacy, Amity University, Noida-201303, Uttar Pradesh, India 2Amity Institute of Phytomedicine and Phytochemistry, Amity Centre for Carbohydrate Research, Amity University, Noida- 201303Uttar Pradesh, India 3Gokaraju Rangaraju College of Pharmacy, Hyderabad, India 4Pharmacopoeia Commission for Indian Medicine and Homoeopathy, Ministry of Ayush, Govt. of India, Ghaziabad-201001, Uttar Pradesh, India 5KIET School of Pharmacy, KIET Group of Institutions, Delhi-NCR, Ghaziabad-201206, Uttar Pradesh, India 6Amity Institute of Phytochemistry and Phytomedicines, Amity University, Noida-201303, Uttar Pradesh, India *Corresponding author e-mail: hkharkwal@amity.edu
Online Published on 06 January, 2022. Abstract Various species of the genus Grewia are reported as glucose-lowering agent besides other diverse pharmacological activity in ethnomedicinal as well as in scientific studies. The present study pursues investigation on the in-vitro anti-diabetic, antioxidant, and cytotoxicity studies of the aqueous-alcoholic and ethyl acetate extract of Grewia abutilifolia leaves. Free radical scavenging activities of the G. abutilifolia were assessed by the 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. The toxicity of extracts was evaluated through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on HepG2 cells, while neurotoxic effects were evaluated by the functional observational battery. Anti-diabetic potential of the extracts was assessed via four in-vitro tests; α-glucosidase inhibitory activity, α-amylase activity, glucose uptake in skeletal muscle cells 6-NBDG (6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose),and GLUT4 uptake activity. In the DPPH assay, extracts showed concentration-dependent antioxidant effects. Ethyl acetate extracts displayed more antioxidant activity compared to aqueous alcoholic extract. Plant extracts did not exhibit any significant cytotoxic effect on the tested cells. Also, the extracts did not inhibit cell proliferation at lower concentration and showed mild anti-proliferative effect at very higher concentration having IC50 values 16 mg/mL and 35.28 mg/mL for aqueous-alcoholic and ethyl acetate extract, respectively. The extracts showed moderate to good anti-diabetic potential based upon the results of the glucosidase, a- amylase activity, glucose uptake and GLUT4 assays. Based upon the outcome of the study,G. abutilifolia was found to possess anti-diabetic and antioxidant potential without any significant toxic effects. Plant can be explored further for its pharmaceutical applications. Top Keywords Toxicity, Pharmaceutical, Hot extraction, MTT assay, α-glucosidase, 6-NBDG, GLUT4 uptake. Top |