Anti-sickling, anti-inflammatory and anti-oxidant potential of Rubia cordifolia L.: A traditionally used medicinal plant of Amarkantak, Madhya Pradesh (India) Mishra Shringika, Sonter Shruti, Dwivedi Manish Kumar, Singh Prashant Kumar* Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, Anuppur-484887, Madhya Pradesh, India *Corresponding author e-mail: prashant.singh@igntu.ac.in
Online published on 23 September, 2021. Abstract Sickle cell disease is a genetic disease affecting millions of people worldwide leading to serious life complications. The current study aims to characterize the traditionally used Rubia cordifolia L. for phytoconstituents, anti-sickling, antiinflammatory and antioxidant potential. Phytochemical screening disclosed the presence of almost all major metabolites to be present in the roots. Maximum percentage of reverse sickling (61.5%) and polymerization inhibition (59%) was observed in the acetone extract. Minimum hemolysis of the RBC was observed in aqueous extract (6.0%). Chloroform extract displayed maximum IC50 (70.6) in DPPH while maximum FRAP value (1.52 μM) was obtained for methanol extract. Acetone extract was rich in TPC (528.3± 2.20 mg GAE/g) TFC (225.8±1.5 mg QE/g) and TTC (1215.6±3.9 mg TAE/g) and displayed maximum protection (85.0%) in membrane stabilization and LOX inhibition (81.5%) while the aqueous extract significantly inhibited XO (71.3%). Overall, the R. cordifolia acetone extract displayed maximum antisickling, anti-inflammatory, membrane stabilization properties and anti-oxidant potential validating the traditional use of R. cordifolia. The results provides us insights for the future development of novel plant based drug/formulations that might help in the treatment or management of SCD. However, bioactivity guided fractionization should be performed for the identification of potent compounds. Top Keywords Sickle cell disease, Rubia cordifolia, Anti-oxidant, Anti-inflammatory, Anti-sickling. Top |