Year : 2017, Volume : 41, Issue : 3
First page : ( 232) Last page : ( 234)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2017 September 1.
Article DOI : 10.5958/0973-970X.2017.00057.8

Histopathological and immunohistochemical studies on cases of chronic respiratory disease in poultry

Brar R.S.², Leishangthem G.D.^1,2, Gadhave P.D.², Singh N.D.^2,*, Banga H.S.², Sodhi S.²

²Department of Veterinary Pathology, College of Veterinary Science, GADVASU, Ludhiana-141004, Punjab, India

¹Animal Disease Research Centre, College of Veterinary Science, GADVASU, Ludhiana-141004, Punjab, India

*Corresponding author: e-mail: drndsingh@gmail.com

Received:  28  March,  2017; Accepted:  18  August,  2017.

Abstract

This study reports histopathological and immunohistochemical findings of chronic respiratory disease in poultry. The necropsy was performed and gross lesions were noted. Grossly, there were pneumonic changes in lung and cloudy air sacs. Microscopically, lung revealed congestion and haemorrhages, lympho-mononuclear cell infiltrates besides necrotic and denuded epithelial cells, suggestive of interstitial pneumonia. Immunohistochemistry revealed Mycoplasma gallisepticum antigen in parabronchiolar and primary bronchiolar epithelial cells and also in inflammatory cells especially macrophages. Thus, in the present study, Mycoplasma gallisepticum infection was diagnosed and confirmed by histopathology and immunohistochemistry.

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Keywords

Histopathology, Immunohistochemistry, Mycoplasma gallisepticum.

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ACKNOWLEDGEMENTS

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Figures

Fig.1.: Lungs: Cloudy air sacs, yellowish to creamy thick coverings on lungs and liver; TopBack

Fig.2.: Lung: Severe congestion and haemorrhages in the interstitium of the parabronchi. H&E ×100; TopBack

Fig.3.: Mycoplasma gallisepticum positive immunolabelling in cytoplasm of epithelial cells of parabronchi (brown colour). IHC ×200; TopBack

Fig.4.: Higher magnification of parabronchi showing Mycoplasma gallisepticum positive immunolocalisation (brown colour) in the cytoplasm of the airway epithelial cells. IHC ×1000; TopBack

Fig.5.: Mycoplasma gallisepticum positive brown colour in the alveolar macrophage within the airway. IHC ×1000. TopBack