Antioxidant activity of ethyl acetate extract of Ipomoea staphylina Mohammad Firdous Sayeed1,*, Amartya Banerjee2, Karmakar Varnita3, Ghosh Arya4 1Professor, Department of Pharmacology, Calcutta Institute of Pharmaceutical Technology & AHS, Uluberia, Howrah-711316, West Bengal, India 2Research Associate, Krish Biotech Research Private Limited, T-1, QK-17, Part, WBIDC, Kalyani, Phase III, West Bengal-741235, India 3Assistant Professor, Eminent College of Pharmaceutical Technology, Barasat, Jagannathpur-700126, West Bengal, India 4Assistant Professor, Department of Pharmaceutical Sciences, Jharkhand Rai University, Ratu Rd, Kamre, Ranchi, Jharkhand-835222, India *Corresponding author e-mail: firdous.oncology@gmail.com
Online Published on 14 April, 2022. Abstract The study aimed to estimate phenolic and iflavonoid content and to assess in vitro antioxidant activity of ethyl acetate extract of Ipomoea staphylina. The total phenolic content of the extract was estimated via Folin-Ciocalteu method and aluminium chloride colorimetric procedure was used to guess the total flavonoid content. DPPH and superoxide scavenging assays were performed to determine in vitro antioxidant activity of I. staphylina extract. The study demonstrated that the scavenging properties of I. staphylina extract against DPPH and superoxide radicals were in a dose depending manner. The extract at 100 μg/ml concentrations has shown maximum DPPH and superoxide radical scavenging effect and the obtained values were comparable with the istandard drug ascorbic acid. The IC50 value of the extract was found to be 35.84 and 30.30 μg/ml in DPPH and superoxide radical scavenging assay, respectively. The extract also contains a moderate quantity of phenolic compounds and flavonoids which are probably responsible for the antioxidant activity. Based on the results found it can be concluded that the ethyl acetate extract of I. staphylina exhibits antioxidant properties probably because of the occurrence of a moderate quantity of phenolic and flavonoid compounds. Top Keywords Ipomoea staphylina, Antioxidant, Phenolic content, Flavonoid content, Dpph, Superoxide, Ascorbic acid. Top |