Variation of diterpene lactone andrographolide, neoandrographolide and andrograpanin in Andrographis paniculata from different locations of India and their content in herbal formulations using HPLC-PDA method Kumar Satyanshu1,,*, Nagar P.S.2, Beena C.3, Nalina L.4, Bishnoi S.K.5, Hussain Tabaruk6, Gupta Moni7, Kumar Ranjeet8, Mirgal Amit9, Deshmukh Amit G.10, Vineeta11, Dhanani Tushar1, Singh Raghuraj1 1ICAR-Directorate of Medicinal and Aromatic Plants Research, Boriavi, Anand, Gujarat, India 2The M.S. University of Baroda, Vadodara, Gujarat, India 3Kerala Agricultural University, Thrissur, Kerala, India 4Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India 5ICAR-NBPGR Regional Station, Ranchi, Jharkhand, India 6Sarat Chandra Singha College of Agriculture, Assam Agricultural University, Dhubri, Assam, India 7Division of Biochemistry, Main Campus, SKUAST-J, Jammu, J&K, India 8Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India 9College of Forestry, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Maharashtra, India 10Dr. Panjabrao Deshmukh Agricultural University, Akola, Maharashtra, India 11Department of Forestry, Faculty of Horticulture, UBKV, Pundibari, Cooch Behar, West Bengal, India *Corresponding author e-mail: satyanshu66@gmail.com
Online published on 23 September, 2021. Abstract Variation in content of three major diterpene lactone andrographolide (AP1), neoandrographolide (AP2) and andrograpanin (AP3) in samples of Andrographis paniculata collected from 15 locations of India were estimated by HPLC-PDA method. These three compounds were also identified and quantified in nine herbal formulations in the form of churna/powder (2 brands), tablets (4 brands) and syrup (3 brands). Wide variations in the concentration of AP1, AP2 and AP3 were recorded in the collected samples as well as herbal formulations containing A. paniculata. The extraction yield of A. paniculata samples collected from different locations of India varied from 10.23 – 21.68% with average extraction yield 14.78%. In collected samples of A. paniculata, AP1 and AP2 were quantified in all samples and their content was recorded in the range of 1.11 ± 0.07 - 4.23 ± 0.1 and 0.03 ± 0.01 – 1.15 ± 0.02%, respectively. AP3 was also detected in all samples, however, it could not get quantified in samples namely APLV 1, APLV 3, APLV 4, APLV 9A, APLV 9B and APLV 11. In herbal formulations, AP1 was quantified in four formulations (APLV 16, APLV 17, APLV 18 and APLV 19). AP2 was quantified in three formulations namely APLV 16, APLV 17 and APLV 18 only. AP3 was quantified in three formulations (APLV 16, APLV 17 and APLV 19) only. Based on the content of AP1, AP2 and AP3 in collected samples, the best location could be selected for the collection of A. paniculata germplasm. Also, from the present investigation, it could be concluded that HPLC-PDA method used here may be used for estimation of AP1, AP2 and AP3 in herbal formulations having A.paniculata as an ingredient. Top Keywords Kalmegh, Quality control, Diterpenoids, Lactone. Top |